Elastin is an important component of extracellular matrix (ECM) in cardiovascular tissue regeneration. We found that human bone marrow stem cells (HBMSC) seeded on porcine small intestinal submucosa (PSIS) bio-scaffolds facilitated higher production of elastin extracellular matrix under flow conditions compared to poly glycolic acid-poly L-lactic acid (PGA-PLLA) synthetic scaffolds. This suggests that extracellular matrices on PSIS bio-scaffolds allowed cells to better communicate and further secrete engineered matrices such as elastin that will be useful in cardiovascular regeneration.